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Takeshi Matsui’s Homepage

松井 毅のホームページ



Matsui T: Endogenous Retroviral-Like Aspartic Protease, SASPase as a Key Modulator of Skin Moisturization.Treatment of Dry Skin Syndrome -The Art and Science of Moisturizers-Edited by Lodén and Maibach, Springer, Part 2: 179-192, 2012.



*Matsui T and Amagai M: Dissecting the formation, structure and barrier function of the stratum corneum. Int. Immunol. 27: 269-80, 2015. (*Corresponding author)


【Research Papers】

Ito Y, Sasaki T, Li Y, Tanoue T, Sugiura Y, Skelly AN, Suda W, Kawashima Y, Okahashi N, Watanabe E, Horikawa H, Shiohama A, Kurokawa R, Kawakami E, Iseki H, Kawasaki H, Iwakura Y, Shiota A, Yu L, Hisatsune J, Koseki H, Sugai M, Arita M, Ohara O, Matsui T, Suematsu M, Hattori M, Atarashi K, Amagai M, Honda K: Staphylococcus cohnii is a potentially biotherapeutic skin commensal alleviating skin inflammation. Cell Rep: 35(4):109052, 2021.


Matsui T*, Kadono-Maekubo N, Suzuki Y, Furuichi Y, Shiraga K, Sasaki H, Ishida A, Sonoko Takahashi S, Okada T, Toyooka K, Sharif J, Abe T, Kiyonari H, Tominaga M, Miyawaki A, Amagai M*: A unique mode of keratinocyte death requires intracellular acidification. Proc. Natl. Acad. Sci. 118:e2020722118, 2021. (*Corresponding author) (Commentary in PNAS by Jessica L. Moorea and Prof. Valentina Greco.) 


Yano T, Tsukita K, Kanoh H, Nakayama S, Kashihara H, Mizuno T, Tanaka H, Matsui T, Goto Y, Komatsubara A, Aoki K, Takahashi R, Tamura A, Tsukita S: A microtubule‐LUZP1 association around tight junction promotes epithelial cell apical constriction. EMBO J. 40:e104712, 2021.


Shiraga K, Urabe M, Matsui T, Kikuchi S, Ogawa Y: Highly precise characterization of the hydration state upon thermal denaturation of human serum albumin using a 65 GHz dielectric sensor. Phys. Chem. Chem. Phys. 22:199468-19479, 2020. (Appeared on the cover)


Shishikura K, Kuroha S, Matsueda S, Iseki H, Matsui T, Inoue A, Arita M: Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic processes in mice. FASEB J. 33:14194-14203, 2020.


Thyssen JP, Jakasa I, Riethmüller C, Schön MP, Braun A, Haftek M, Fallon PG, Wróblewski J, Jakubowski H, Eckhart L, Declercq W, Koppes S, Engebretsen KA, Bonefeld C, Irvine AD, Keita-Alassane S, Simon M, Kawasaki H, Kubo A, Amagai M, Matsui T, Kezic S: Filaggrin expression and processing deficiencies impair corneocyte surface texture and stiffness in mice. J Invest. Dermatol. 140:615-623, 2019. (Appeared on the cover)


Usui K, Kadono N, Furuichi Y, Shiraga K, Saitou T, Kawasaki H, Toyooka K, Tamura H, Kubo A, Amagai M, Matsui T*: 3D in vivo imaging of the keratin filament network in the mouse stratum granulosum: filaggrin as an extrinsic keratin-bundling factor. J Dermatol. Sci. 94:346-349, 2019. (*Corresponding author) (Appeared on the cover)


Wang J, Sekai M, Matsui T, Fujii Y, Matsumoto M, Takeuchi O, Minato N, Hamazaki Y: Hassall’s corpuscles with cellular-senescence features maintain IFNα production through neutrophils and pDC activation in the thymus. Int. Immunol. 31:127-139, 2018.


Hawkins RFW, Patenaude A, Dumas A, Jain R, Tesfagiorgis Y, Kerfoot S, Matsui T, Gunzer M, Pelletier M, Larochelle C, Poubelle PE, Valliéres L: ICAM1+ neutrophils promote chronic inflammation via ASPRV1 in B cell–dependent autoimmune encephalomyelitis. J. Clin. Invest. Insight 2:e96882, 2017.


Yano T, Matsui T, Tamura A, Uji M, Tsukita S: The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK. J. Cell Biol. 203:605-14, 2014.


Sandilands A, Brown SJ, Goh CS, Pohler E, Wilson NJ, Campbell LE, Miyamoto K, Kubo A, Irvine AD, Thawer-Esmail F, Munro CS, McLean WH, Kudoh J, Amagai M, Matsui T: Mutations in the SASPase gene (ASPRV1) are not associated with atopic eczema or clinically dry skin. J. Invest. Dermatol. 132:1507-10, 2012.


Ono H, Imoto I, Kozaki K, Tsuda H, Matsui T, Kurasawa Y, Muramatsu T, Sugihara K, Inazawa J: SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation. Oncogene 31:4923-34, 2012.


Watanabe K, Oochiai TKikuchi SKumano TMatsui TKoji M, Yasukawa S, Nakamori S, Sasako M, Yanagisawa A, Otsuji E: Dermokine expression in intraductal papillary-mucinous neoplasm and invasive pancreatic carcinoma Anticancer Res 32:4405-12, 2012.


Matsui T*, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, Tanahashi S, Ichinose S, Imoto I, Inazawa J, Kudoh J, Amagai M: SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing. EMBO Mol. Med. 3:320-333, 2011. (*Corresponding author)


Muramatsu T, Imoto I, Matsui T, Kozaki K, Haruki S, Sudol M, Shimada Y, Tsuda H, Kawano T, Inazawa J: YAP is a candidate oncogene for esophageal squamous cell carcinoma. Carcinogenesis 32, 389-398, 2011.


Tagi T#, Matsui T#, Kikuchi S, Hoshi S, Ochiai T, Kokuba Y, Kinoshita-Ida Y, Kisumi-Hayashi F, Morimoto K, Imai T, Imoto I, Inazawa J, Otsuji E: Dermokine as a novel biomarker for early-stage colorectal cancer. J. Gastroenterol. 45:1201-11, 2010. (#Equal contribution)


Haruki S, Imoto I, Kozaki K, Matsui T, Kawachi H, Komatsu S, Muramatsu T, Shimada Y, Kawano T, Inazawa J: Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous-cell carcinoma. Carcinogenesis 31:1027-36, 2010.


Konishi H, Kikuchi S, Ochiai T, Ikomai H, Kubota T, Ichikawa D, Fujiwara H, Okamoto K, Sakakura C, Sonoyama T, Kokuba Y, Sasaki H, Matsui T, Otsuji E: Latrunculin A has a strong anti-cancer effect in a peritoneal dissemination model of human gastric cancer in mice. Anticancer Res. 29:2091-7, 2009.


Nojima H, Adachi M, Matsui T, Okawa K, Tsukita S, Tsukita S: IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade. Nature Cell Biol. 10:971-978, 2008.


Katsuno T, Umeda K, Matsui T, Hata M, Tamura A., Itoh M, Takeuchi K, Fujimori T, Nabeshima YI, Noda T, Tsukita S, Tsukita S: Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells. Mol. Biol. Cell 19:2465-2475, 2008.


Umeda K, Ikenouchi J, Katahira-Tayama S, Furuse K, Sasaki H, Nakayama M, Matsui T, Tsukita S, Furuse M, Tsukita S: ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation. Cell 126:741-54, 2006.


Matsui T*, Kinoshita-Ida Y, Hayashi-Kisumi F, Hata M, Matsubara K, Chiba M, Katahira-Tayama S, Morita K, Miyachi Y, Tsukita S: Mouse homologue of skin-specific retroviral-like aspartic protease (SASPase) involved in wrinkle formation. J. Biol. Chem. 281:27512-25, 2006. (*Corresponding author)


Komiya S, Shimizu M, Ikenouchi J, Yonemura S, Matsui T, Fukunaga Y, Liu H, Endo F, Tsukita S, Nagafuchi A: Apical membrane and junctional complex formation during simple epithelial cell differentiation of F9 cells. Genes Cells 10:1065-80, 2005.


Tamura A, Kikuchi S, Hata M, Katsuno T, Matsui T, Hayashi H, Suzuki Y, Noda T, Tsukita S, Tsukita S: Achlorhydria by ezrin knockdown: defects in the formation/expansion of apical canaliculi in gastric parietal cells. J. Cell Biol. 169:21-8, 2005.


Umeda K, Matsui T, Nakayama M, Furuse K, Sasaki H, Furuse M, Tsukita S: Establishment and characterization of cultured epithelial cells lacking expression of ZO-1.  J. Biol. Chem. 279:44785-94, 2004.


Matsui T, Hayashi-Kisumi F, Kinoshita Y, Katahira S, Morita K, Miyachi Y, Ono Y, Imai T, Tanigawa Y, Komiya T, Tsukita S: Identification of novel keratinocyte-secreted peptides dermokine-α/-β and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1. Genomics 84:384-97, 2004.


Kikuchi S, Hata M, Fukumoto K, Yamane Y, Matsui T, Tamura A, Yonemura S, Yamagishi H, Keppler D, Tsukita S, Tsukita S: Radixin deficiency causes conjugated hyperbilirubinemia with loss of Mrp2 from bile canalicular membranes. Nat. Genet. 31:320-5, 2002.


Yonemura S, Matsui T, Tsukita S, Tsukita S: Rho-dependent and -independent activation mechanisms of ezrin/radixin/moesin proteins: an essential role for polyphosphoinositides in vivo. J. Cell Sci. 115:2569-80, 2002.


Ishikawa H, Tamura A, Matsui T, Sasaki H, Hakoshima T, Tsukita S, Tsukita S: Structural conversion between open and closed forms of radixin: low-angle shadowing electron microscopy. J. Mol. Biol. 310:973-8, 2001.


Hamada K, Shimizu T, Matsui T, Tsukita S, Tsukita S, Hakoshima T: Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of the adhesion protein ICAM-2. Acta Crystallogr. D. Biol. Crystallogr. 57:891-2, 2001.


Hamada K, Seto A, Shimizu T, Matsui T, Takai Y, Tsukita S, Tsukita S, Hakoshima T: Crystallization and preliminary crystallographic studies of RhoGDI in complex with the radixin FERM domain. Acta Crystallogr. D. Biol. Crystallogr. 57:889-90, 2001.


Hamada K, Shimizu T, Matsui T, Tsukita S, Hakoshima T: Structural basis of the membrane-targeting and unmasking mechanisms of the radixin FERM domain. EMBO J. 19:4449-62, 2000.


Hamada K, Matsui T, Tsukita S, Tsukita S, Hakoshima T: Crystallographic characterization of the membrane-binding domain of radixin. Acta Crystallogr. D. Biol. Crystallogr. 56:922-3, 2000.


Matsui T, Yonemura S, Tsukita S, Tsukita S: Activation of ERM proteins in vivo by Rho involves phosphatidyl-inositol 4-phosphate 5-kinase and not ROCK kinases. Curr. Biol. 9:1259-62, 1999.


Maeda M, Matsui T, Imamura M, Tsukita S, Tsukita S: Expression level, subcellular distribution and rho-GDI binding affinity of merlin in comparison with ezrin/radixin/moesin proteins. Oncogene 18:4788-97, 1999.


Hayashi K, Yonemura S, Matsui T, Tsukita S: Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues. J. Cell Sci. 112:1149-58, 1999.


Matsui T, Maeda M, Doi Y, Yonemura S, Amano M, Kaibuchi K, Tsukita S, Tsukita S: Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association. J. Cell Biol. 140:647-57, 1998.


Matsui T, Amano M, Yamamoto T, Chihara K, Nakafuku M, Ito M, Nakano T, Okawa K, Iwamatsu A, Kaibuchi K: Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J. 15:2208-16, 1996.


Amano M, Mukai H, Ono Y, Chihara K, Matsui T, Hamajima Y, Okawa K, Iwamatsu A, Kaibuchi K: Identification of a putative target for Rho as the serine-threonine kinase protein kinase N. Science 271:648-50, 1996.


Yamamoto T, Matsui T, Nakafuku M, Iwamatsu A, Kaibuchi K: A novel GTPase-activating protein for R-Ras. J. Biol. Chem. 270:30557-61, 1995.



松井毅:ドライスキンによる皮膚バリア障害の機序:フィラグリン、タイトジャンクション『エビデンスに基づくアトピー性皮膚炎治療 新しい潮流』編集椛島健治、宮地良樹、中山書店、第三章:67-75, 2019.



松井 毅:皮膚バリアとアレルギー『臨床免疫・アレルギー科』76:93-99, 2021.


葛野菜々子、古市祐樹、松井毅:表皮バリアの形成機構『臨床免疫・アレルギー科』 68:196-202, 2017.


松井毅:陸上脊椎動物の皮膚の適応進化と内在性レトロウイルス『ウイルス』 66:31-38, 2016.


松井毅:表皮顆粒層に特異的に発現するプロテアーゼSASPaseと角質層の保湿機構『臨床免疫・アレルギー科』 58:305-312, 2012.


松井毅:脊椎動物陸上進出の謎と美容-表皮顆粒層に特異的に発現するレトロウイルス様アスパラギン酸プロテアーゼSASPaseの研究を通して-『日本美容皮膚科学会誌』 21:277-287, 2011.


松井毅、月田早智子:ERMタンパク質のRhoによる機能制御 アクチンフィラメント-細胞膜相互作用における役割『細胞工学』 17:1856-64, 1998.


土井喜宣、松井毅、月田早智子:ERM(ezrin/radixin/moesin)タンパク質を介したアクチンフィラメント-細胞膜相互作用と細胞内情報伝達『実験医学』 16:89-97, 1998.


山本隆晴、松井毅、貝淵弘三:その他のRasサブファミリー(R-Ras, Ral)『実験医学』 14:163-166, 1996.